Skip to main content
Addgene
Showing: 541 - 560 of 2136 results

  2. pFBD-BirA_PRPF4:1-522

    Plasmid
    #210896
    Purpose
    Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    PRPF4:M1-E522 (PRPF4 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210896-plasmid-map-sequence-id-424523

    Enlarge

  3. pFBD-BirA_RFWD3:425-774

    Plasmid
    #210884
    Purpose
    Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    RFWD3:S425-E774 (RFWD3 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210884-plasmid-map-sequence-id-424500

    Enlarge

  4. pFBD-BirA_WDR91:392-747

    Plasmid
    #210880
    Purpose
    Insect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR91:P392-A747 (WDR91 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210880-plasmid-map-sequence-id-424497

    Enlarge

  7. pCAM9

    Plasmid
    #78560
    Purpose
    pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.
    Depositor
    James Kaper
    Article
    Merrell et al J Bacteriol. 2001 May;183(9):2746-54.
    Insert
    ompU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid
    #229681
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Halo, ALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229681-plasmid-map-sequence-id-457127

    Enlarge

  9. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

    Enlarge

  11. pLZRS-TrkC-mCherry

    Plasmid
    #182949
    Purpose
    To express the rat TrkC fused in the C-terminus with mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    TRk-C-mCherry (Ntrk3 Synthetic, Rat)
    Use
    Retroviral and Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182949-plasmid-map-sequence-id-360115

    Enlarge

  12. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Anne Mason, Ross MacGillivray
    Article
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Use
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

    Enlarge

  13. pCMV-BRAF-REG-T241P-Halo

    Plasmid
    #202549
    Purpose
    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.
    Depositor
    Deborah Morrison
    Article
    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e
    Insert
    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Threonine 241 mutated to proline
    Promoter
    CMV
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    202549-plasmid-map-sequence-id-401163

    Enlarge

Showing: 541 - 560 of 2136 results