We narrowed to 1,090 results for: cas12a

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pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Plasmid

#195543

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR-Zeo

Plasmid

#158709

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Publication

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Expression

Bacterial

Promoter

Hsp60

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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EC64420

Plasmid

#209517

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW303-AcrVA5

Plasmid

#115657

Purpose

Expresses AcrVA5 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag

Plasmid

#236385

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236364

Purpose

Overexpression of dLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236367

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag

Plasmid

#236365

Purpose

Overexpression of dLbCas12aRVRR in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12aRVRR

Use

CRISPR and Lentiviral

Mutation

G532R, K538V, Y542R, K595R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236376

Purpose

Overexpression of HyperdFnCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdFnCas12a

Use

CRISPR and Lentiviral

Mutation

E184R, T279R, D340R, D409R

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236375

Purpose

Overexpression of dFnCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dFnCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140229

Purpose

Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Publication

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

AntiCRISPR protein AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z13

Plasmid

#120056

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Publication

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EC70202

Plasmid

#209463

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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