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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Publication

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Publication

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-neoT2AFlagCDK10wt-W

Plasmid

#246511

Purpose

Lentiviral vector expressing human Flag-tagged gRNA-resistant human CDK10

Depositor

Kosuke Yusa

Publication

Islam et al Cell Death Dis. 2026 Jun 11;17(1):737. d

Insert

CDK10 (CDK10 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Silent mutations are added to make the cDNA resis…

Promoter

Human EF1a promoter

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-neoT2AFlagCDK10me3-W

Plasmid

#246512

Purpose

Lentiviral vector expressing human Flag-tagged gRNA-resistant mutant human CDK10 (D181N)

Depositor

Kosuke Yusa

Publication

Islam et al Cell Death Dis. 2026 Jun 11;17(1):737. d

Insert

CDK10 (CDK10 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Kinase-dead mutation (D181N) and silent mutations…

Promoter

Human EF1a promoter

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499R

Plasmid

#203574

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

Depositor

Abhishek Chakraborty

Publication

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5- tagged

Expression

Mammalian

Mutation

silent point mutations introduced to make it resi…

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

Plasmid

#242750

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-wt

Plasmid

#184269

Purpose

Expresses shRNA resistant GFP-RepoMan

Depositor

Lienhard Schmitz

Publication

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

3xHA and GFP

Expression

Mammalian

Mutation

changed nucleotides 723-747, 1488-1509, 1524-1548…

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499/500R

Plasmid

#203575

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

Depositor

Abhishek Chakraborty

Publication

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

silent point mutations to make resistant shRNA 49…

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Publication

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaACAbox

Plasmid

#242753

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaHbox

Plasmid

#242752

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-MUT- deltaSNORA13

Plasmid

#242751

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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