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pαH-S-GSAS/D614G

Plasmid

#164566

Purpose

SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-GSAS-D614G variant)

Use

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GAD67 scFv [L127/8]

Plasmid

#190509

Purpose

Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)

Use

Tags

Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Tiam1 [L130/1R]

Plasmid

#177476

Purpose

Mammalian Expression Plasmid of anti-Tiam1 (Mouse). Derived from hybridoma L130/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Tiam1 (Mus musculus) recombinant mouse monoclonal antibody (Tiam1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.8 Na+ channel [N134/12R]

Plasmid

#188203

Purpose

Mammalian Expression Plasmid of anti-Nav1.8 Na+ channel (Rat). Derived from hybridoma N134/12

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.8 Na+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Scn10a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mCarmine

Plasmid

#183464

Purpose

To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mCarmine

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Shank2 [N23B/6R]

Plasmid

#177519

Purpose

Mammalian Expression Plasmid of anti-Shank2 (Rat). Derived from hybridoma N23B/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Shank2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Shank2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

Plasmid

#171952

Purpose

All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized, truncated at amino acid 114 with…

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-NrCAM [N343/26R]

Plasmid

#177534

Purpose

Mammalian Expression Plasmid of anti-NrCAM (Mouse). Derived from hybridoma N343/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NrCAM (Mus musculus) recombinant mouse monoclonal antibody (Nrcam Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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BKbeta2 K+ channel scFv [N53/32]

Plasmid

#190561

Purpose

Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-WRN [N428/12R]

Plasmid

#206575

Purpose

Mammalian Expression Plasmid of anti-WRN (Human). Derived from hybridoma N428/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-WRN (Homo sapiens) recombinant Mouse monoclonal antibody (WRN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-beta variant

Plasmid

#194598

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

Depositor

Ben Murrell

Article

Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

Insert

CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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sgCRY1

Plasmid

#176511

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY1-2 (Cry1, Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Expression

Mutation

Promoter

U6, hSyn

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

Plasmid

#206006

Purpose

Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

Depositor

Xue Han

Article

Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

Insert

SomArchon-mTagBFP2-P2A-CoChR-Kv2.1

Use

AAV

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N6/38R]

Plasmid

#182117

Purpose

Mammalian Expression Plasmid of anti-VAChT (Human). Derived from hybridoma N6/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Homo sapiens) recombinant mouse monoclonal antibody (SLC18A3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSico_U6-Pan-PGC1a sgRNA

Plasmid

#165426

Purpose

Expression of gRNA against human Total PGC-1a variants

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against human Total PGC-1a variants (PPARGC1A Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM-Asn209Ala_M18

Plasmid

#91733

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Asn209 changed to Ala (EU numbering)

Promoter

hEF1-HTLV prom

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/4/8 [N245/36R]

Plasmid

#177522

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat). Derived from hybridoma N245/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody (Tarp Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pIGF1_P2 Promoter-FLuc

Plasmid

#154267

Purpose

Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

IGF-1 P2 Promoter (IGF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human IGF-1 P2 Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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