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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-T158M

Plasmid

#179410

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(T158M) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD T158M

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-63-SFFV-T2A-BlaR-polyA-R11a

Plasmid

#179894

Purpose

Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Expression

Bacterial and Mammalian

Promoter

SFFV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-61-SFFV-T2A-PuroR-polyA-R11a

Plasmid

#179892

Purpose

Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Expression

Bacterial and Mammalian

Promoter

SFFV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

Plasmid

#177145

Purpose

Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO_S.C.FLAG_NGFR

Plasmid

#158332

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.FLAG

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.C.NWS_NGFR

Plasmid

#158333

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.NWS

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.C.HA_NGFR

Plasmid

#158334

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO_S.C.AU1_NGFR

Plasmid

#158335

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.AU1

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.C.VSVg_NGFR

Plasmid

#158336

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.FLAG.NWS_NGFR

Plasmid

#158342

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.FLAG.NWS

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.FLAG.HA_NGFR

Plasmid

#158343

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.FLAG.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.FLAG.AU1_NGFR

Plasmid

#158344

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.FLAG.AU1

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.NWS.HA_NGFR

Plasmid

#158346

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.NWS.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.NWS.AU1_NGFR

Plasmid

#158347

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.NWS.AU1

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.HA.AU1_NGFR

Plasmid

#158349

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.HA.AU1

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_S.HA.VSVg_NGFR

Plasmid

#158350

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.HA.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_Tag.VSVg.S.Ollas_NGFR

Plasmid

#158309

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

Tag.VSVg.S.Ollas

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_Ollas.FLAG.NWS_NGFR

Plasmid

#158321

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

Ollas.FLAG.NWS

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_Ollas.FLAG.HA_NGFR

Plasmid

#158322

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

Ollas.FLAG.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_NWS.Ollas.HA_NGFR

Plasmid

#158325

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

NWS.Ollas.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_NWS.Ollas.VSVg_NGFR

Plasmid

#158327

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

NWS.Ollas.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HA.Ollas.VSVg_NGFR

Plasmid

#158329

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.Ollas.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.C.VSVg_NGFR

Plasmid

#158287

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.C.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.FLAG.NWS_NGFR

Plasmid

#158293

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.FLAG.NWS

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.FLAG.VSVg_NGFR

Plasmid

#158296

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.FLAG.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.NWS.HA_NGFR

Plasmid

#158297

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.NWS.HA

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.NWS.VSVg_NGFR

Plasmid

#158299

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.NWS.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_HSV.HA.VSVg_NGFR

Plasmid

#158301

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HSV.HA.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_Tag.FLAG.S.Ollas_NGFR

Plasmid

#158305

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

Tag.FLAG.S.Ollas

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge