We narrowed to 15,732 results for: LEA

1. BB44_pmc.DonorR5.TS

   Plasmid

   #199223

   Purpose

   Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
   

   Use

   Gene targeting donor plasmid

   Expression

   Mammalian

   Promoter

   Human PGK1 gene promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgPBRM1-2- LentiCRISPRv2

   Plasmid

   #107404

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF12J

   Plasmid

   #42943

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   EN+ Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   AgeI-BclI fragment swapped with nt 1927-3708 from…

   Promoter

   CMV MIE, L1 5'UTR

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA1 Flag ATXN1[30Q]

   Plasmid

   #33236

   Depositor

   Huda Zoghbi

   Article

   Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 29 amino acids (27 Q…

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

   Plasmid

   #205319

   Purpose

   All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISTfu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNAintron-CHIKV-Structural polyprotein

   Plasmid

   #215698

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-E2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-JNK1

    Plasmid

    #86830

    Purpose

    mammalian expression of GFP-JNK1

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK1 (MAPK8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dominant negative Hsp90 beta

    Plasmid

    #22480

    Depositor

    William Sessa

    Article

    Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

    Insert

    Dominant negative Hsp90 (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-HA-MRE11-H63S

    Plasmid

    #82035

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H63S MRE11 mutant

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-EGxxFP-Cetn1

    Plasmid

    #50717

    Purpose

    Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    Cetn1 (Cetn1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. AY27_pU6.gRNA.CLYBL

    Plasmid

    #199238

    Purpose

    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. pyogenes gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas12j-8-puro-crRNA

    Plasmid

    #194965

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hsyn-GRAB_Ado1.0med

    Plasmid

    #153286

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-GRAB_OT1.0

    Plasmid

    #185384

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK/CMV and minP

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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