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Addgene

We narrowed to 34,404 results for: CaS

Showing: 5601 - 5620 of 34404 results
  1. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid
    #221843
    Purpose
    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Insert
    EGFP
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Insert
    Target sequence
    Use
    Target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pHochTypeIII

    Plasmid
    #214039
    Purpose
    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6
    Depositor
    Insert
    Cas10
    Use
    CRISPR
    Tags
    Expression
    Mutation
    WT
    Promoter
    T7 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pEcNucC

    Plasmid
    #214050
    Purpose
    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system
    Depositor
    Insert
    EcNucC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    lacUV5 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5601 - 5620 of 34404 results