We narrowed to 9,085 results for: mel

1. pcDNA3-Flag-Cortactin

   Plasmid

   #74476

   Purpose

   This plasmid was constructed to study cortactin interactions

   Depositor

   J Thomas Parsons

   Article

   Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

   Insert

   Cortactin (Cttn Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

   Plasmid

   #163928

   Purpose

   Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RDX-mCherry

   Plasmid

   #214476

   Purpose

   Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   RDX (RDX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Sequence matches cDNA clone MGC:48283 IMAGE:52844…

   Promoter

   CMV

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75154

   Purpose

   Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabePuro-ApoECDS

   Plasmid

   #42949

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

   Insert

   Apolipoprotein E (APOE Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. psiCheck-ApoECDS

   Plasmid

   #41848

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

   Insert

   ApoE CDS (APOE Human)
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-TWIN-STREP-FLAG-NPRL2

   Plasmid

   #164985

   Purpose

   NPRL2 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL2 (NPRL2 Human)
   

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRFP703-Clathrin

    Plasmid

    #79997

    Purpose

    Clathrin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Clathrin (CLTB Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYO1273

    Plasmid

    #235746

    Purpose

    Expression of FL human EGFP-ATG14L in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Insert

    ATG14L (ATG14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAWPSG-Ptet-nCas9-BE

    Plasmid

    #195739

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptet(Tetracycline inducible protein)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Insect

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-TWIN-STREP-FLAG-NPRL3

    Plasmid

    #164984

    Purpose

    NPRL3 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL3 (NPRL3 Human)
    

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-Flag-Narg2

    Plasmid

    #49247

    Purpose

    mammalian expression of flag-tagged human Narg2

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Narg2 (NARG2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pFUGW-GtACR1_C102A-EYFP

    Plasmid

    #119196

    Purpose

    Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin GtACR1 step-function mutant C102A
    

    Use

    Lentiviral

    Tags

    EYFP

    Mutation

    changed cysteine 102 to alanine

    Promoter

    Ubiquitin

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #219426

    Purpose

    Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Promoter

    EF-1alpha

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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