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pTW324

Plasmid

#115955

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 End

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW064

Plasmid

#115951

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 ORF Dest

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GP803

Plasmid

#164547

Purpose

Expresses Smo-FLAG K434, 444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

All three Lysines in cytoplasmic loop three of Sm…

Promoter

GgCryD1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP802

Plasmid

#164546

Purpose

Expresses Smo-FLAG K348, 360R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K348, 360R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Both the Lysines in cytoplasmic loop two of Smoo…

Promoter

GgCryD1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcihygrosynDAT

Plasmid

#19992

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

synDAT (SLC6A3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
P315-FBP_10

Plasmid

#139701

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_66_neg_Control

Plasmid

#130567

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_62_neg_Control

Plasmid

#130565

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_35_neg_Control

Plasmid

#130564

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_92

Plasmid

#130540

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_71

Plasmid

#130533

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_62

Plasmid

#130530

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_61

Plasmid

#130529

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_18_neg_Control

Plasmid

#130560

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_19

Plasmid

#130520

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_84

Plasmid

#130536

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_80

Plasmid

#130535

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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