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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCA1.332 pDUOTK1-L1

Plasmid

#162351

Purpose

Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

Depositor

Alistair McCormick

Article

Gale et al Front Microbiol. 2021 Jan 15;11:624011.

Inserts

eYFP
LacZ alpha
mTagBFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCO708

Plasmid

#139745

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-15

Plasmid

#117044

Purpose

Constitutive integrase cassette - Bxb1-Tp901-Int5-Int7

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Tp901-Int5-Int7

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJL024

Plasmid

#122863

Purpose

Binary expression vector containing 35S:MS2-p300:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-p300:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSW178 - pXPP

Plasmid

#115987

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Alexis Maizel

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pXPP

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSW614 - pREV

Plasmid

#115989

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSW454 - pUFO

Plasmid

#115978

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pUFO

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSW179 - pML1

Plasmid

#115977

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST BmOr2

Plasmid

#73926

Purpose

UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

BmOr2

Expression

Yeast

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRB31

Plasmid

#72868

Purpose

template for N-terminal 1x Strep tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

single Strep tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pKF292

Plasmid

#72856

Purpose

template base vector for N-terminal tagging, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for Nterm-tag templates

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNO201

Plasmid

#249344

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridia

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Insert

group II intron and ItrA

Use

E. coli - clostridium shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3HA

Plasmid

#221048

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3FLAG

Plasmid

#221045

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xFLAG epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSK-Sasg

Plasmid

#214350

Purpose

Expresses cloning backbone for SlugCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 tracrRNA

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

PQS1

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi Bla mAmetrine

Plasmid

#183257

Purpose

Stable expression of blue fluorescent mAmetrine protein in a Sleeping Beauty transposon vector.

Depositor

Alexander McLellan

Article

He et al Int J Mol Sci. 2020 Dec 4;21(23). pii: i

Insert

mAmetrime

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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