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pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Publication

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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K44A HA-dynamin 2 pcDNA3.1

Plasmid

#34685

Depositor

Sandra Schmid

Insert

dynamin 2ba

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCSCre

Plasmid

#31132

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

Cre recombinase (cre bacteriophage P1)

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Publication

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Expression

Mammalian

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Publication

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PX408 Francisella tularensis subsp. novicida Cas9

Plasmid

#68705

Purpose

Francisella tularensis subsp. novicida Cas9

Depositor

Feng Zhang

Insert

FtCas9

Use

CRISPR

Tags

HA-NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Rabbit GFP Cav 2.2 pMT2

Plasmid

#58737

Purpose

expression of GFP tagged rabbit Cav2.2 in mammalian cells

Depositor

Annette Dolphin

Publication

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cav2.2 (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Publication

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP2

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAM1

Plasmid

#51401

Purpose

Expresses truncated version of NLL-MetRS from endogenous MetG promoter

Depositor

David Tirrell

Publication

Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Insert

MetRS (metG E coli)

Mutation

L13N, Y260L, H301L; Please note that a truncated …

Promoter

MetG

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL2B-HIF1A prom

Plasmid

#40172

Depositor

Alex Minella

Publication

Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

Insert

HIF1A promoter fragment (HIF1A Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV:DsRed(FRT)GFP

Plasmid

#31128

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

DsRed(FRT)GFP

Use

Flp/frt

Expression

Mammalian

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-tdTomato-CNA35

Plasmid

#61606

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

tdTomato and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CMV-hEAAT1

Plasmid

#32813

Depositor

Susan Amara

Insert

EAAT1 (SLC1A3 Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pETM33_Nsp5_Mpro

Plasmid

#156475

Purpose

Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Publication

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Publication

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EGF-bio-His

Plasmid

#53340

Purpose

Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

EGF (EGF Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles