We narrowed to 14,557 results for: NTS;

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pGWB424

Plasmid

#74818

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway-compatible entry vector

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRIF Flag

Plasmid

#41550

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

Trif (TICAM1 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE347

Plasmid

#153228

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-J6-NbGP41-sfGFP-VP64-GB1-J9

Plasmid

#202007

Purpose

MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

NbGP41-sfGFP-VP64-GB1

Use

Synthetic Biology; Moclo cloning vector

Tags

GB1 and sfGFP

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-RPL18

Plasmid

#238253

Purpose

For overexpression of mCherry-RPL18

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-RPL18

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211842

Purpose

Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Inserts

LRR-NEL
GAL4-VP16-GR

Use

Synthetic Biology

Tags

HA, Myc, and mCherry

Expression

Plant

Promoter

6xUAS-35Smini and pUBQ10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pGWB540

Plasmid

#74874

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2mito

Plasmid

#158726

Purpose

Plant mitoTALEN vector assembly, Destination vector

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

mitochondrial presequence in front of attR1 in pk7WG2

Expression

Plant

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB504

Plasmid

#74846

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Article

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

Plasmid

#86424

Purpose

PCR tagging module with yeast codon optimized mTurquoise2

Depositor

Sue Jaspersen

Article

Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

Insert

mTurquoise2

Expression

Yeast

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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ArcLight-Q239

Plasmid

#36856

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

simian CMV

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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