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Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralTagsExpressionMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Human, Synthetic)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9
Plasmid#162758Purposeexpress miCas9DepositorInsertmiCas9
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationContains Brex27 sequence range is 4958-5065PromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP618_αGCN4-KRAB-CO
Plasmid#211782PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertaGCN4-mCherry
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect mCherr…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-FNIP1
Plasmid#72293PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-5102HRasCT(Picchu)
Plasmid#209917PurposeA FRET biosensor for tyrosine kinase activityDepositorInsert5102HRasCT
UseTagsYPet and nTurquoise-GLExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TurboID-CRaf
Plasmid#226168PurposeLentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.DepositorInsertMyc-TurboID-CRaf (RAF1 Human)
UseLentiviralTagsEGFP-IRES-BlastR and Myc-TurboIDExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorUseTagsExpressionMutationPromoterpTrcAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 TadDE
Plasmid#193846PurposeExpress TadDE (with SaCas9) in mammalian cellsDepositorInsertTadDE with SaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLC242-GFP-metap2
Plasmid#100518PurposeExpresses GFP tagged metap2 in mammalian cells to make lentivirusDepositorInsertMetap2 (METAP2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOD1_P2A_Hygro_Barcode
Plasmid#120464PurposeBarcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH4
Plasmid#168980PurposeAMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…TagsExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Amp
Plasmid#207998PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
px330-CD4
Plasmid#136938PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDHDepositorInsertsgRNA targeting CD4 (CD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FNTA-FLAG
Plasmid#86858PurposeTo express FNTA with a FLAG tag at C terminus.DepositorInsertFNTA (FNTA Human)
UseTagsFLAGExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only