We narrowed to 14,036 results for: TIM

1. VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanR

   Plasmid

   #235113

   Purpose

   Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

   Depositor

   Oscar Ramos

   Article

   Guyot et al bioRxiv 2025.10.29.680610

   Inserts

   Asf1B (ASF1B Human)
   IP4
   

   Use

   Synthetic Biology; Quantitative bacterial two-hyb…

   Tags

   cI_E34P-rpoA

   Expression

   Bacterial

   Promoter

   LtetO and lpp+lacUV5

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. VN1264 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_WeaKRBS_TIR51_KanR

   Plasmid

   #235114

   Purpose

   Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

   Depositor

   Oscar Ramos

   Article

   Guyot et al bioRxiv 2025.10.29.680610

   Inserts

   Asf1B (ASF1B Human)
   IP3mut3A
   

   Use

   Synthetic Biology; Quantitative bacterial two-hyb…

   Tags

   cI_E34P-rpoA

   Expression

   Bacterial

   Promoter

   LtetO and lpp+lacUV5

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. VN1263 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_WeaKRBS_TIR51_KanR

   Plasmid

   #235115

   Purpose

   Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

   Depositor

   Oscar Ramos

   Article

   Guyot et al bioRxiv 2025.10.29.680610

   Inserts

   Asf1B (ASF1B Human)
   IP1
   

   Use

   Synthetic Biology; Quantitative bacterial two-hyb…

   Tags

   cI_E34P-rpoA

   Expression

   Bacterial

   Promoter

   LtetO and lpp+lacUV5

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-huCof R21Q-mRFP

   Plasmid

   #245964

   Purpose

   Expression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Insert

   cofilin 1 (cfl1) (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   R21Q

   Promoter

   CMV

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. huCof K22Q-mRFP

   Plasmid

   #245965

   Purpose

   Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Insert

   cofilin 1 (cfl1) (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   K22Q

   Promoter

   CMV

   Available Since

   Oct. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5-MLLn-CAGEn-NES (Nrdj1)

   Plasmid

   #241412

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   MLLn-CAGEn-NES (Nrdj1) (KMT2A Human, Synthetic)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-MLLn-CAGEn-NES (Npu)

   Plasmid

   #241411

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   MLLn-CAGEn-NES (Npu) (KMT2A Human, Synthetic)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-AMLn-CAGEn-NES (Npu)

   Plasmid

   #241407

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   AMLn-CAGEn-NES (Npu) (RUNX1 Human, Synthetic)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-20XUAS-IVS-Syn21-Positron2-p10

   Plasmid

   #239082

   Purpose

   Gal4 activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2
   

   Expression

   Insect

   Mutation

   R78K N81D D92N W178F

   Promoter

   hsp70

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10

    Plasmid

    #239081

    Purpose

    LexA activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Expression

    Insect

    Mutation

    R78K N81D D92N W178F

    Promoter

    hsp70

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Promoter

    Chicken Beta-actin

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

    Plasmid

    #224788

    Purpose

    SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crCoV20, crCoV21, crCoV24
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. DG SaCas9 GFP V3

    Plasmid

    #226961

    Purpose

    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DG SaCas9 puro V3

    Plasmid

    #226960

    Purpose

    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDG458 eSp(1.1) V3

    Plasmid

    #226964

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-CD28-mTFP1

    Plasmid

    #223638

    Purpose

    Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mTFP1

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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