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pCEP4-myc-EphB1

Plasmid

#200981

Purpose

Mammalian expression plasmid for myc-tagged EphB1

Depositor

Erik Procko

Publication

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EphB1 (EPHB1 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB4

Plasmid

#200983

Purpose

Mammalian expression plasmid for myc-tagged EphB4

Depositor

Erik Procko

Publication

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB6

Plasmid

#200984

Purpose

Mammalian expression plasmid for myc-tagged EphB6

Depositor

Erik Procko

Publication

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EphB6 (EPHB6 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-Akt-STOPS

Plasmid

#175010

Purpose

Akt-STOPS targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Publication

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2A-mCherry-Akt-STOPS

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE3_IVT

Plasmid

#177015

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Publication

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE3

Use

CRISPR; Vector for in-vitro transcription

Promoter

T3 promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Publication

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE4

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mGFP-APEX2

Plasmid

#171936

Purpose

Reporter for correlated light and electron microscopy.

Depositor

David Berson

Publication

Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

Insert

mGFP-APEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9675-CBE

Plasmid

#163007

Purpose

plasmid expressing CBE

Depositor

Ervin Welker

Publication

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Publication

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWY

Plasmid

#112906

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-SOMA

Plasmid

#118935

Purpose

Used to express the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Publication

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana

Expression

Bacterial

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Publication

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (334-449)

Plasmid

#87859

Purpose

Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Publication

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

fragment from aa 334 to 449

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GIV-FLAG S245A (SA)

Plasmid

#86095

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Publication

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

S245A

Promoter

CMV

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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GIV-FLAG S245D (SD)

Plasmid

#86096

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Publication

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

S245D

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Publication

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23119

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX/p14ARF

Plasmid

#78761

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Publication

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-NSD3-short W284A

Plasmid

#72551

Purpose

expresses 3*FLAG tagged human NSD3-short with W284A mutation

Depositor

Christopher Vakoc

Publication

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

W284A

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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