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Showing: 1401 - 1450 of 11379 results

  1. pUC19-G9WGU4

    Plasmid
    #103112
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    G9WGU4(Cas9 coding gene from Oenococcus kitaharae DSM 17330)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103112-plasmid-map-sequence-id-201871

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  2. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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  5. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid
    #131112
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131112-plasmid-map-sequence-id-257485

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  6. pcDNA4/TO-ORF66 C289A-2xStrep

    Plasmid
    #131120
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 289 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131120-plasmid-map-sequence-id-257523

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  7. pcDNA4/TO-ORF66 C244A-2xStrep

    Plasmid
    #131121
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 244 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131121-plasmid-map-sequence-id-257526

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  11. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid
    #223112
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223112-plasmid-map-sequence-id-447635

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  16. pOPINRSF

    Plasmid
    #41128
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41128-plasmid-map-sequence-id-54712

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  19. pOPINGS

    Plasmid
    #41121
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    March 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    41121-plasmid-map-sequence-id-54678

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  20. pOPINJB

    Plasmid
    #41123
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    March 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    41123-plasmid-map-sequence-id-54682

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  21. V21 CS2+ RFPbb

    Plasmid
    #17112
    Depositor
    Randall Moon
    Type
    Empty backbone
    Use
    Xenopus/mammalian/avian/zebrafish
    Tags
    RFP
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  22. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  23. pOPINE-3C-HALO7

    Plasmid
    #41126
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41126-plasmid-map-sequence-id-54701

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  24. pTAU-CN-DM

    Plasmid
    #111230
    Purpose
    yeast expression vector
    Depositor
    Ulrich Stelzl
    Insert
    ccdB-cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111230-plasmid-map-sequence-id-211521

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  25. pTAU-C-DM

    Plasmid
    #111231
    Purpose
    yeast expression vector
    Depositor
    Ulrich Stelzl
    Insert
    ccdB-cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111231-plasmid-map-sequence-id-214073

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  26. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41125-plasmid-map-sequence-id-54698

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  27. pOPINE-3C-CD4

    Plasmid
    #41127
    Depositor
    Ray Owens
    Insert
    CD4
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41127-plasmid-map-sequence-id-54705

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  28. pUC57KAN-20XALFA

    Plasmid
    #233112
    Purpose
    20X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233112-plasmid-map-sequence-id-466717

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  29. plex_307-3xflag-ccdb-blasticidin

    Plasmid
    #201123
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201123-plasmid-map-sequence-id-396322

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  30. plex_307-3xflag-ccdb-puro

    Plasmid
    #201122
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and puro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201122-plasmid-map-sequence-id-395513

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  31. plex_307-EYFP-ccdB-zeo

    Plasmid
    #201129
    Purpose
    pLEX_307 plasmid with N-terminal EYFP tag and zeo marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201129-plasmid-map-sequence-id-400052

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  32. plex_307-mKate-ccdB-zeo

    Plasmid
    #201126
    Purpose
    pLEX_307 plasmid with N-terminal mKate tag and zeo marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    mKate
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201126-plasmid-map-sequence-id-397035

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  33. pBP-C_NanoLuc

    Plasmid
    #204112
    Purpose
    Type 0 CDS part
    Depositor
    Mikhail Shapiro
    Insert
    NanoLuc luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204112-plasmid-map-sequence-id-413909

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  34. plex_307-EYFP-ccdB-g418

    Plasmid
    #201128
    Purpose
    pLEX_307 plasmid with N-terminal EYFP tag and g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201128-plasmid-map-sequence-id-396318

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  35. plex_307-mKate-ccdB-g418

    Plasmid
    #201125
    Purpose
    pLEX_307 plasmid with N-terminal mKate tag and g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    mKate
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201125-plasmid-map-sequence-id-397034

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  36. CSII-CAG-EGFP

    Plasmid
    #214112
    Purpose
    Mammalian continuous expression
    Depositor
    Sho Yoshimatsu
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214112-plasmid-map-sequence-id-423582

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  37. EYFP-TRAF1

    Plasmid
    #111200
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Use
    Tags
    EYFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111200-plasmid-map-sequence-id-212973

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  39. pColdII-SIRPT1-Chlorocebus sabaeus Sacsin

    Plasmid
    #78112
    Purpose
    Nt His-tag Chlorocebus sabaeus Sacsin 86-1375
    Depositor
    Kalle Gehring
    Insert
    Sacsin Chlorocebus sabaeus 86-1375
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78112-plasmid-map-sequence-id-145660

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  40. Dm_msi_RRM1

    Plasmid
    #61112
    Purpose
    Express His tagged Drosophila musashi RRM1
    Depositor
    Sean Ryder
    Insert
    Drosophila musashi RBD
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    stop codon after first RRM domain
    Promoter
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61112-plasmid-map-sequence-id-99119

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  41. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  42. KBB611

    Plasmid
    #185112
    Purpose
    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 repeats 4&5-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185112-plasmid-map-sequence-id-368078

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  43. EGFP-AR

    Plasmid
    #111215
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    AR (Ar Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111215-plasmid-map-sequence-id-264404

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  44. TNFR2-GFP

    Plasmid
    #111207
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111207-plasmid-map-sequence-id-213090

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  45. TNFR2-YFP

    Plasmid
    #111210
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111210-plasmid-map-sequence-id-212997

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  47. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  48. pAAV-CMV-Slu2Cas9-puro

    Plasmid
    #232112
    Purpose
    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232112-plasmid-map-sequence-id-462527

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  49. 2OQK

    Plasmid
    #60112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Cp-PF11_0447:MAC01Z-A10:C31333
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Nov. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1401 - 1450 of 11379 results