Addgene

We narrowed to 18,315 results for: Tec

Showing: 1401 - 1450 of 18315 results

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ER-mEGFP

Plasmid

#182876

Purpose

Localization: Endoplasmic Reticulum

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

ER-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-Backbone(NG)

Plasmid

#27963

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NG)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pKmK.P2

Plasmid

#125035

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.T4

Plasmid

#125056

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

6xHis and Venus

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTS1019_Tier3(PB)

Plasmid

#169649

Purpose

Tier-3 vector for stable integration of up to three cassettes via piggyBac transposase (ITR-A1::A2::A3-ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P7

Plasmid

#125040

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TEF1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-FALSE

Plasmid

#89689

Purpose

pCAG promoter driving no CDS

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA1

Plasmid

#75388

Purpose

sgRNA1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pTHSSe_51

Plasmid

#109249

Purpose

p15A BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMevB

Plasmid

#17819

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pCMycDam

Plasmid

#59218

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Mutation

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src-myc

Plasmid

#25933

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-Src (Src Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P1

Plasmid

#125034

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PGK1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Use

Tags

See map

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-T7-YhjH

Plasmid

#79165

Purpose

Expresses YhjH under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

yhjH

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.T1

Plasmid

#125053

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

INU1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P15

Plasmid

#125048

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TDH3 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.T5

Plasmid

#125057

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PGK1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P16

Plasmid

#125049

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

FBA1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoofy56

Plasmid

#122008

Purpose

Mammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10/none and TwinStrep

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoofy40

Plasmid

#44006

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10-Stop-OneStrep-Stop-S-Stop-CBP-Stop-HPC4-Sto…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMG-RIS

Plasmid

#225132

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsy4

Plasmid

#44252

Depositor

Adam Arkin, Stanley Qi

Article

Qi et al Nat Biotechnol. 2012 Oct;30(10):1002-6.

Insert

csy4 gene

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-STS

Plasmid

#35949

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

Stilbene synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

LacPMod

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL60004

Plasmid

#117519

Purpose

rbcS-E9 terminator Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GCTT:E9t:CGCT:BsaI

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-Backbone(NN)

Plasmid

#27961

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NN)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2038

Plasmid

#39396

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2039

Plasmid

#39397

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
p_SNIPR_target_WT

Plasmid

#139465

Purpose

T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_WT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWp7

Plasmid

#72730

Purpose

pFUGW-UBCp-RFP-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEP-EGFPin

Plasmid

#60961

Purpose

PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

eGFP-Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p_SNIPR_target_mut_36

Plasmid

#139466

Purpose

T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_mut36

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEZ10

Plasmid

#81135

Purpose

pACSA_J23113_tetR PEZ3_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GGE0029

Plasmid

#120730

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Type

Empty backbone

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-Backbone(NI)

Plasmid

#27962

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NI)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtRPS5a

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P3

Plasmid

#125036

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

ENO1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-Backbone(HD)

Plasmid

#27964

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(HD)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
LBJJ491

Plasmid

#117513

Purpose

AtYAO promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pYAO:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtYAO

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
dBrainbow

Plasmid

#29327

Depositor

Julie Simpson

Article

Hampel et al Nat Methods. 2011 Feb 6. ():.

Insert

UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-Zyxin-6

Plasmid

#58148

Purpose

Localization: Focal Adhesions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Zyxin (ZYX Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 1401 - 1450 of 18315 results

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