We narrowed to 15,681 results for: LEA

1. pcDNA3.1-MUC2 D1 with cleavable His tag

   Plasmid

   #181813

   Purpose

   Expresses human MUC2 D1 with cleavable His tag, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCS2-FlipGFP(Casp3 cleavage seq)

   Plasmid

   #124432

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq)
   

   Use

   Zebrafish (good expression)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

   Plasmid

   #204971

   Purpose

   Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   raACR
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CaMKII

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

   Plasmid

   #204970

   Purpose

   Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   MsACR1
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CaMKII

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. paavCAG-JxON-pre-mGRASP-mCerulean

   Plasmid

   #51903

   Purpose

   JxOn-pre

   Depositor

   Jinhyun Kim

   Article

   Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

   Insert

   presynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

   Plasmid

   #92400

   Purpose

   Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and membrane tag

   Expression

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2plus-mCerulean 156-239-GGGS

   Plasmid

   #162616

   Purpose

   Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mCerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   156-239

   Promoter

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Virgina Zakian, Jean-Baptiste Boulé

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Use

   Tags

   histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDUET-1-alpha-synuclein-mCerulean3-His6

   Plasmid

   #110061

   Purpose

   For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   6 x His and mCerulean3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUSE-Src-YF-UniRapR-mCerulean-myc

    Plasmid

    #45381

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-YF-UniRapR-mCerulean-myc
    

    Use

    Tags

    Myc, Myr, and mCerulean

    Expression

    Mammalian

    Mutation

    changed mSrc Tyrosine 529 to Phenylalanine

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92402

    Purpose

    Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Kalyviotis et al (2020) Behavioral and Neural Genetics of

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and No tag, cytosolic expression

    Expression

    Mutation

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. paavCAG-JxOFF-pre-mGRASP-mCerulean

    Plasmid

    #51900

    Purpose

    JxOFF-pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    presynaptic mGRASP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBRY-nuclear mCherry-IRES-PURO

    Plasmid

    #52409

    Purpose

    Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    nuclear mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSems leader-ALFA-mXFP-TpoR

    Plasmid

    #222945

    Purpose

    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    Thrombopoietin receptor (26-635) (MPL Human)
    

    Use

    Tags

    ALFAtag, Ig k-chain leader sequence, and mXFP

    Expression

    Mammalian

    Mutation

    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 mCerulean LIC cloning vector (6E)

    Plasmid

    #30128

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92401

    Purpose

    Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and membrane tag

    Expression

    Mutation

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid

    #79882

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUSE-Src-KD-UniRapR-mCerulean-myc

    Plasmid

    #45382

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-KD-UniRapR-mCerulean-myc
    

    Use

    Tags

    mCerulean and myc

    Expression

    Mammalian

    Mutation

    mSrc R388D (kinase dead), Y529F

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only