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p3xFLAG-CMV-14-Mfn1-T562A

Plasmid

#191976

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562A (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562A

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562D

Plasmid

#191977

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562D (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562D

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

Plasmid

#62945

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

Plasmid

#62944

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340D

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564A

Plasmid

#191978

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564A (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T564A

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564D

Plasmid

#191979

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564D (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T564D

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165888

Purpose

Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (L) (FA)

Plasmid

#170627

Purpose

For the mammalian expression of mLOV2GIVe lit-mimicking mutation I539E and the GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L) (FA)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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FAM165B_pCSdest

Plasmid

#53860

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SMIM11 (FAM165B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pAG496

Plasmid

#226750

Purpose

Expresses exogenous MTCH2

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Daxx/pEGFP

Plasmid

#119021

Purpose

Express GFP-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

death-domain associated protein (Daxx) (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDL14

Plasmid

#106901

Purpose

pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
mutS
lacI
rhaR
rhaS

Use

Tags

Expression

Bacterial

Mutation

Lysine 622 was replaced by Alanine

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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