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pCX:α4ΔICD-EC4-GA

Plasmid

#208512

Purpose

ddGFP-A-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420-G91E_S

Plasmid

#147469

Purpose

Bacterial Expression of CeSMG5_1-420-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420-G91E (smg-5 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-PTC_E

Plasmid

#146191

Purpose

Mammalian Expression of BGG-PTC

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-PTC (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-WT_E

Plasmid

#146192

Purpose

Mammalian Expression of BGG-WT

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-WT (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395-G80E_S

Plasmid

#147476

Purpose

Bacterial Expression of CeSmg7_1-395-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395-G80E (smg-7 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDB4961

Plasmid

#174015

Purpose

pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Vps15 (ppk19 Fission Yeast)

Use

Tags

13Myc, Vps15

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4953

Plasmid

#174020

Purpose

pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Use

Tags

GFP, Atg6

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4658

Plasmid

#174017

Purpose

pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg8

Use

Tags

GFP, Atg8

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4958

Plasmid

#174014

Purpose

pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Use

Tags

CFP, Atg6

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4948

Plasmid

#174019

Purpose

pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Vps38

Use

Tags

Pil1, mCherry, Vps38

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4946

Plasmid

#174018

Purpose

pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg14 (atg14 Fission Yeast)

Use

Tags

Pil1, mCherry, Atg14

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB4925

Plasmid

#174016

Purpose

pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg38(161-190)

Use

Tags

Pil1, mCherry, Atg38(161-190)

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-BvCYP76AD1

Plasmid

#162529

Purpose

Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

CYP76AD1

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-MjcDOPA5GT

Plasmid

#162530

Purpose

Mirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

cDOPA5GT

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Nectin-2

Plasmid

#170295

Purpose

A knock-out vector for the mouse Nectin-2

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Nectin-2 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Necl-5

Plasmid

#170294

Purpose

A knock-out vector for the mouse Necl-5

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Necl-5 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

Plasmid

#127409

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127399

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

Plasmid

#127408

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

Plasmid

#127406

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge