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Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM159
Plasmid#173215PurposeExpresses dCas9-sfGFP10DepositorInsertdCas9-sfGFP10M2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM80
Plasmid#173173PurposeHA-dCas9DepositorInsertdCas9
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-8x-gRNA-eGFP
Plasmid#60718PurposeeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activationDepositorInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9D10A
Plasmid#128161PurposePtet-controlled expression of S. pyogenes Cas9D10A variantDepositorInsertSp Cas9
UseTagsExpressionBacterialMutationD10APromoterPtetAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426*-LbSgH
Plasmid#136992PurposeHelper plasmid for LbCpf1 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2480
Plasmid#160080PurposeZ. mobilis CRISPRi, promoter C, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2132
Plasmid#160075PurposeZ. mobilis CRISPRi, promoter B, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only