We narrowed to 1,322 results for: pET28a

1. pET28a-His6-GFP-UROD-N367A

   Plasmid

   #236742

   Purpose

   Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   eGFP-Uroporphyrinogen decarboxylase human N367A (UROD Human, Synthetic)
   

   Use

   Tags

   His6 and eGFP

   Expression

   Bacterial

   Mutation

   N367A

   Promoter

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10

   Plasmid

   #226351

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

   Plasmid

   #226352

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1

   Plasmid

   #226353

   Purpose

   Expression of titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-VHH-ddFLN4-HIS-ybbR

   Plasmid

   #169137

   Purpose

   E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   VHH
   

   Use

   Tags

   6x His tag, ddFLN4 tag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a- mCherry-FIVAR-HIS-ybbR

   Plasmid

   #169138

   Purpose

   E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   mCherry
   

   Use

   Tags

   6x Histag, FIVAR tag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-SdrG-B1-ELP-HIS-ybbR

   Plasmid

   #169133

   Purpose

   E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

   Plasmid

   #214339

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (Il18 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ENGD29DDEN_N36Y_H41E

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SznF H407A H409A H448A

    Plasmid

    #139566

    Purpose

    expressed SznF H407A H409A H448A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF H407A H409A H448A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pet28a His6-HSPB7 (zebrafish) C49S

    Plasmid

    #110071

    Purpose

    Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 49 to Serine

    Promoter

    T7

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

    Plasmid

    #117981

    Purpose

    SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B1
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

    Plasmid

    #117982

    Purpose

    SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

    Plasmid

    #117983

    Purpose

    SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge