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pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

Plasmid

#185915

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

Plasmid

#226989

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1a to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

Plasmid

#226991

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2a to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

Plasmid

#226987

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A6 to create OPA1-R194G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

Plasmid

#226986

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A7 to create OPA1-R194G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

Plasmid

#226992

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2b to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

Plasmid

#226990

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1b to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

Plasmid

#226988

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A9 to create OPA1-R194G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Expression

Mutation

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Sox2-IRES-Neo

Plasmid

#13462

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Sox2 (Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3G124-HilA

Plasmid

#153323

Purpose

Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xPax7gRNA

Plasmid

#224571

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Expression

Mutation

Codon optimized

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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