We narrowed to 1,631 results for: prep

1. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

   Plasmid

   #229790

   Purpose

   Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEFL-HAx2-TEADiv2

   Plasmid

   #228873

   Purpose

   Expression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

   Insert

   TEADiv2
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEFL-EGFP-TEADiv2

   Plasmid

   #228872

   Purpose

   Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

   Insert

   TEADiv2
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHPHS926

   Plasmid

   #228237

   Purpose

   Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Inserts

   mCherry
   Beta globin
   

   Use

   Tags

   3xFLAG, HA, and PuroR

   Expression

   Mammalian

   Mutation

   PTC39

   Promoter

   TRE3GV and none

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. OSCA1.2-K435L-L438K-peGFP-N1

   Plasmid

   #228587

   Purpose

   Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K435L, L438K

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Efg1-ΔDBD-Y115S/Y132S/Y139S

   Plasmid

   #216408

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y115S/Y132S/Y139S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 115, 132 and 139 to Serine, and …

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-EF1a_NKX2-1_HD_160-223AA

   Plasmid

   #221489

   Purpose

   NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deletion of 1-159AA N-terminal and 224-371AA C-te…

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRFP670-C1-Nir1-LNS2

   Plasmid

   #220085

   Purpose

   high affinity PA biosensor with far-red fluorophore

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   iRFP670-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL475

    Plasmid

    #219503

    Purpose

    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNeonGreen-N1-Nir1-613-630

    Plasmid

    #220088

    Purpose

    N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    GGSGG-NeonGreen

    Expression

    Mammalian

    Mutation

    amino acids 613-630

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Efg1-ΔDBD-A51P/A56P/Q443P

    Plasmid

    #216402

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56 and Glutamine 443 to Proli…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Efg1-ΔDBD-Y4S/Y8S/Y16S

    Plasmid

    #216405

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y4S/Y8S/Y16S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 4, 8 and 16 to Serine, and delet…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Efg1-ΔDBD-Y64S/Y66S/Y69S

    Plasmid

    #216406

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y64S/Y66S/Y69S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 64, 66 and 69 to Serine, and del…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Efg1-ΔDBD-Y101S/Y104S/Y106S

    Plasmid

    #216407

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y101S/Y104S/Y106S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 101, 104 and 106 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Efg1-ΔDBD-Y401S/Y404S/Y409S

    Plasmid

    #216409

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y401S/Y404S/Y409S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 401, 404 and 409 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Efg1-ΔDBD-Y450S/Y452S/Y456S

    Plasmid

    #216410

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y450S/Y452S/Y456S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 450, 452 and 456 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Efg1-ΔDBD-Y478S/Y482S/Y484S

    Plasmid

    #216411

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y478S/Y482S/Y484S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 478, 482 and 484 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1114H

    Plasmid

    #200640

    Purpose

    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

    Depositor

    Omar Akbari

    Article

    Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

    Insert

    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST39-pNL29

    Plasmid

    #91696

    Purpose

    Expresses Mega GVs in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

    Insert

    Mega GV gene cluster (from pNL29) in pST39 backbone
    

    Use

    Tags

    No tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge