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pMS02

Plasmid

#230066

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2321 (Cloning backbone, bRNA + recombinase]

Plasmid

#247249

Purpose

Cloning backbone for cloning bridge recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mito-BFP

Plasmid

#49151

Purpose

visualization of mitochondria

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

mitochondrial targeting signal (COX4 Budding Yeast)

Tags

BFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet2

Plasmid

#218368

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Gm-SceI-pheS

Plasmid

#111609

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID_G118R

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCap-SG5-Hgy

Plasmid

#227580

Purpose

Capture plasmid with hygromycin resistance and pSG5 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHelp

Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Tags

VP16 AD (413-490)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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