We narrowed to 15,732 results for: LEA

1. pDisplay-GRAB_OTmut

   Plasmid

   #185385

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E WT

   Plasmid

   #110123

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Oct. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBK.014

   Plasmid

   #187215

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNHT7::RPL15::hGluc

   Plasmid

   #186768

   Purpose

   An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Gaussia luciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1352wUV2 F2bbs kan GCANNNAGGk

   Plasmid

   #18752

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-PKM2-G415R

   Plasmid

   #122693

   Purpose

   Express His-tagged PKM2 (G415R) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   G415R

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIG-Flag-RIPK1-D324A

   Plasmid

   #102678

   Purpose

   retroviral expression plasmid for RIPK1-D324A

   Depositor

   Hasam Habelhah

   Article

   Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Retroviral

   Tags

   Flag and IRES-GFP

   Expression

   Mammalian

   Mutation

   CHANGE Glutamate 324 to Alanine

   Promoter

   unknown

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX402 caPHF2

   Plasmid

   #91701

   Purpose

   inducible lentiviral expression of constitutively active PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   caPHF2; S757D, S899D, S954D, S1056D

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pETNKI-strepII-3C-LIC-kan

    Plasmid

    #108706

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PD-L1-miSFIT-4x

    Plasmid

    #124684

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

    Plasmid

    #13617

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 65, Thr 69 and Ser70 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1352wUV2 F3bbs kan NNNTGGGCGk

    Plasmid

    #18756

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNHT7::RPL15::sNluc2

    Plasmid

    #186763

    Purpose

    An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-GCaMP3-44

    Plasmid

    #91780

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-44
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1352wUV2 F1bbs kan GCGTGGNNN

    Plasmid

    #18753

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDsRed2-C1-Ahi1

    Plasmid

    #30495

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid

    #70666

    Purpose

    Reduced the expression of gamma-tubulin in murine cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    

    Use

    U6 promoter

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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