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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pED-hIRE1(I642G)

Plasmid

#16263

Depositor

Peter Walter

Article

Lin et al Science. 2007 Nov 9. 318(5852):944-9.

Insert

IRE1 (ERN1 Human)

Expression

Mammalian

Mutation

isoleucine to glycine mutation at residue 642

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAGM55297

Plasmid

#153213

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-eGFP

Plasmid

#157734

Purpose

mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-eGFP

Tags

Flag-His8

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AMPK-β2-MYC

Plasmid

#40606

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP-mCherry

Plasmid

#27763

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP-monomeric Cherry (tandem)

Expression

Mammalian

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCytERM_mScarlet-H_N1

Plasmid

#85067

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR-tagBFP-PSM-dTOMATO

Plasmid

#100604

Purpose

Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

dTOMATO-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pG4-CMV-luc

Plasmid

#46322

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

5xGal4

Use

Luciferase

Tags

CMV promoter and luciferase

Expression

Mammalian

Mutation

five copies of a multimerized GAL4 (5×GAL4) site …

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 MDM2 S186D

Plasmid

#16235

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pCI HA NEDD4L S382A S468A

Plasmid

#27003

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

S382A S468A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv [Preprint]. 2024 Nov 1:2024.11.0

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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KG#901

Plasmid

#110936

Purpose

Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-GFP

Plasmid

#40123

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-H2A-Akt-STOPS

Plasmid

#175010

Purpose

Akt-STOPS targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-mCherry-Akt-STOPS

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN

Plasmid

#73365

Purpose

Expresses myc tagged XPLN in mammalian cells

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-H2A-TORCAR

Plasmid

#175016

Purpose

TORCAR targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-TORCAR

Tags

Histone H2A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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