We narrowed to 8,324 results for: gnal

1. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

   Plasmid

   #181851

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lysine 128 in the DnaJB1-PKAcat fusion changed to…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

   Plasmid

   #192785

   Purpose

   Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and mXFPe

   Expression

   Mammalian

   Mutation

   mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214278

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
   Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   Cdc42-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

   Plasmid

   #156415

   Purpose

   kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mTagBFP2

   Expression

   Mutation

   mutant KRAS4A-G12D

   Promoter

   kdrl

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

   Plasmid

   #181853

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Human, Synthetic)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

   Plasmid

   #156416

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mScarlet-I

   Expression

   Mutation

   mutant KRAS4A-G12V

   Promoter

   kdrl

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

   Plasmid

   #180468

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   KRAS4A (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   V5-mScarlet-I

   Expression

   Mutation

   Promoter

   kdrl

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

   Plasmid

   #156406

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Expression

   Mutation

   mutant KRAS4A-G12V

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-FLAG-MKK6-K82M

    Plasmid

    #21583

    Depositor

    John Kyriakis

    Article

    Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lysine 82 to methionine

    Promoter

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pLEX_307-SOD1E133Δ

    Plasmid

    #83445

    Purpose

    Mammalian expression of SOD1E133Δ.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133Δ

    Promoter

    EF-1α

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid

    #156414

    Purpose

    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    mutant KRAS4B-G12V

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSOD1E133K-AcGFP1

    Plasmid

    #83442

    Purpose

    Expresses SOD1E133K in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133K

    Promoter

    CMV

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX

    Plasmid

    #232264

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-GID1-Cdc42/N12 (CDC42 Human)
    Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX (CDC42 Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX

    Plasmid

    #232265

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-GID1-Cdc42/N12 (CDC42 Human)
    Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX (CDC42 Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(Q63L)-PYL-mVenus-CAAX

    Plasmid

    #232266

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-ABI-RhoA/N12 (RHOA Human)
    RhoA/13C(Q63L)-PYL-mVenus-CAAX (RHOA Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(T19N)-PYL-mVenus-CAAX

    Plasmid

    #232267

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-ABI-RhoA/N12 (RHOA Human)
    RhoA/13C(T19N)-PYL-mVenus-CAAX (RHOA Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    RhoA-T19N

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTR01F/hFGFR1b-V5

    Plasmid

    #236012

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR1b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR1b (FGFR1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRP-BRG1-HA-SmBiT

    Plasmid

    #241815

    Purpose

    Expresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system

    Depositor

    Ethan Lee

    Article

    Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

    Insert

    BRG1 (SMARCA4 Human)
    

    Use

    Tags

    HA and SmBiT

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214279

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge