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pHR: pPGK PYL1-sfGFP-HP1Adel72

Plasmid

#174113

Purpose

Expression of PYL1-sfGFP-HP1Adel72

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1Adel72

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET - FnR062.Y78V

Plasmid

#84801

Purpose

bacterial expression of FnR.0.6.2-Y78V protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78V

Tags

6xHis

Expression

Bacterial

Mutation

Y78V

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78A

Plasmid

#84784

Purpose

bacterial expression of FnR.0.6.2-Y78A protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78A

Tags

6xHis

Expression

Bacterial

Mutation

Y78A

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78C

Plasmid

#84785

Purpose

bacterial expression of FnR.0.6.2-Y78C protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78C

Tags

6xHis

Expression

Bacterial

Mutation

Y78C

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78E

Plasmid

#84787

Purpose

bacterial expression of FnR.0.6.2-Y78E protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78E

Tags

6xHis

Expression

Bacterial

Mutation

Y78E

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78L

Plasmid

#84793

Purpose

bacterial expression of FnR.0.6.2-Y78L protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78L

Tags

6xHis

Expression

Bacterial

Mutation

Y78L

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78M

Plasmid

#84794

Purpose

bacterial expression of FnR.0.6.2-Y78M protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78M

Tags

6xHis

Expression

Bacterial

Mutation

Y78M

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78N

Plasmid

#84795

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Expression

Bacterial

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78Q

Plasmid

#84797

Purpose

bacterial expression of FnR.0.6.2-Y78Q protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78Q

Tags

6xHis

Expression

Bacterial

Mutation

Y78Q

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78T

Plasmid

#84800

Purpose

bacterial expression of FnR.0.6.2-Y78T protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78T

Tags

6xHis

Expression

Bacterial

Mutation

Y78T

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#149339

Purpose

expresses M.Ocy1ORF8430P DNA methyltransferase (M.SpeI ortholog) in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.Ocy1ORF8430P

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-tagRFP-IMBB2.4-pUC57-mini

Plasmid

#149343

Purpose

expresses tagRFP fluorescent protein in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

tagRFP

Expression

Bacterial

Promoter

rhamnose

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RGS8

Plasmid

#140585

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RGS8

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-GTPBP2

Plasmid

#140586

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA GTPBP2

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVd2-Dr-TrkB

Plasmid

#127955

Purpose

Myr DrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-3xSAG-Dr-hel4-TrkB

Expression

Mammalian

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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spoTD297A_NS1

Plasmid

#100915

Purpose

expresses spoTD297A (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Mutation

Changed D 297 to A

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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