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pET15b p97-His D592Amb

Plasmid

#169022

Purpose

Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His

Expression

Bacterial

Mutation

D592TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-EGFP-p16 shRNA

Plasmid

#164919

Purpose

Expresses EGFP cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
CDKN2A (CDKN2A Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM L368G

Plasmid

#169985

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, L368G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM R394E

Plasmid

#169987

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, R394E

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F405G

Plasmid

#169988

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F405G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F370G

Plasmid

#169986

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G + S297A

Plasmid

#169974

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R + S297A

Plasmid

#169968

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E + S297A

Plasmid

#169972

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM E274R

Plasmid

#169977

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

Plasmid

#169955

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

Plasmid

#169957

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, F405G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

Plasmid

#169951

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R, S297A

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3486 (YCp LEU2 Rpb1 F1492A)

Plasmid

#91808

Purpose

Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed phenylalanine 1492 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3487 (YCp LEU2 Rpb1 S1493A)

Plasmid

#91809

Purpose

Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed serine 1493 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3488 (YCp LEU2 Rpb1 P1494A)

Plasmid

#91810

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1494 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3508 (YCp LEU2 Rpb1 Y1473A)

Plasmid

#91811

Purpose

Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed tyrosine 1473 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3509 (YCp LEU2 Rpb1 T1471A)

Plasmid

#91812

Purpose

Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed threonine 1471 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3511 (YCp LEU2 Rpb1 P1472A)

Plasmid

#91813

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1472 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3567 (pET151_Spt6 1247-1451 R1282H)

Plasmid

#91816

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed arginine 1282 to histidine; deleted resid…

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3495 (pET151_Spt6 1247-1451 K1435A)

Plasmid

#91817

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1435 to alanine; deleted residues …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pQC hKGA.S286A V5-His IRES G418

Plasmid

#110391

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C E490R

Plasmid

#164027

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E490R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C R495E

Plasmid

#164028

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R495E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C D511K

Plasmid

#164029

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, D511K

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C Y527A

Plasmid

#164030

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C NLRP1 CARD G1467C

Plasmid

#164009

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

G1467C

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge