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pCO601

Plasmid

#139733

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_p35S:NnHisps:t35S

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pNR188

Plasmid

#79543

Purpose

Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSYC-102

Plasmid

#74790

Purpose

pCS4+-NLS-EGFP-T2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-T2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy31

Plasmid

#43993

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6 and OneStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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KHK

Plasmid

#25532

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KHK (KHK Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-dHeFSpCas9-VP64-6xHis

Plasmid

#92119

Purpose

Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCerulean-N1 Cyclin B1 (1147)

Plasmid

#61852

Purpose

mammalian expression of Cyclin B1 fused to Cerulean

Depositor

Jonathon Pines

Insert

Cyclin B1 (CCNB1 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pAM1706

Plasmid

#40242

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

PpsbAI

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF

Plasmid

#26262

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB601NL3F10H

Plasmid

#141287

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Gateway Cloning and Luciferase

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ339

Plasmid

#117501

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-IndHom-Tol2-TetR-cryaa-dsRed

Plasmid

#73204

Purpose

BAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

dsRed

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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