We narrowed to 9,109 results for: control

1. pDH18

   Plasmid

   #83803

   Purpose

   YFP-CFP positive FRET control; his selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   YFP-CFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p663-UBC-V5-BioID-STOP_IDG-K

   Plasmid

   #158163

   Purpose

   Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-BioID
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

   Plasmid

   #160630

   Purpose

   TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

   Depositor

   Diego Orzaez

   Insert

   PhyBVP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-hcRed-V5-TurboID_IDG-K

   Plasmid

   #135238

   Purpose

   Gateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   hcRed-V5-TurboID
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZNpF-deleted

   Plasmid

   #245372

   Purpose

   Negative control, luciferase cassette deleted

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDual_dsCas9_Puro_hs_NC_chr1

   Plasmid

   #214688

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Puromycin resistance cassette has silent mutation…

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV/CBA::EGFP-P2A-tC3/54aa-FLAG

   Plasmid

   #129479

   Purpose

   AAV2 transfer plasmid for truncated C3 (54 aa) tagged with FLAG with EGFP under control of the CBA promoter

   Depositor

   Robert Gross

   Insert

   truncated (AA148-201) exoenzyme C3 from C. botulinum, P2A, EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV/CBA::EGFP-P2A-tC3-FLAG

   Plasmid

   #129477

   Purpose

   AAV2 transfer plasmid for truncated C3 tagged with FLAG with EGFP under control of the CBA promoter

   Depositor

   Robert Gross

   Insert

   FLAG-tagged (C-terminus) truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-79aa-ActA-pEGFP-C1

   Plasmid

   #166763

   Purpose

   To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid

    #135260

    Purpose

    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-dsRed
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Or46a-Gal4

    Plasmid

    #63179

    Purpose

    Expresses Gal4 under control of the Drosophila melanogaster Or46a odorant receptor promoter

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

    Insert

    Or46a promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pC0043-PspCas13b-gRNA non target

    Plasmid

    #223698

    Purpose

    gRNA control for dPspCas13b-FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for luciferase control
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid

    #79649

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AIMTOR A757

    Plasmid

    #140829

    Purpose

    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR A757 (ULK1 Human)
    

    Use

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid

    #79650

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    isoleucine 679 changed to lysine

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET.SUMO eIF4G1 1-200

    Plasmid

    #158791

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (codon optimized)
    

    Use

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-mFzd8

    Plasmid

    #102870

    Purpose

    Expresses mouse Fzd8 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T to C substitution at nucleotide 1134, C to T su…

    Promoter

    EF1A

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid

    #158792

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon) codon optimized
    

    Use

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-KDM6A-M1190T/E1335D

    Plasmid

    #203581

    Purpose

    Expresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.

    Depositor

    William Kaelin, Abhishek Chakraborty

    Article

    Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

    Insert

    KDM6A (KDM6A Human)
    

    Use

    Lentiviral

    Tags

    HA-tagged

    Expression

    Mammalian

    Mutation

    Changed Methionine 1190 Threonine and Glutamic Ac…

    Promoter

    CMV

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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