We narrowed to 9,085 results for: mel

1. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMito-miRFP703

   Plasmid

   #80000

   Purpose

   Mitochondria labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   cytochrome C oxidase subunit VIII (COX8A Human)
   

   Tags

   miRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-CLASP2 512-650

   Plasmid

   #24409

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (512-650) (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant with only MT plus end trac…

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5-HA-p300-DY1399

   Plasmid

   #89095

   Purpose

   mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300-D1399Y mutant (EP300 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p300-DY1399

   Promoter

   SV40

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-hLin28B

   Plasmid

   #26358

   Depositor

   George Daley

   Article

   Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

   Insert

   lin-28 homolog B (LIN28B Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE8.13-m

   Plasmid

   #136296

   Purpose

   expresses ABE8.13-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.13-m
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-Ldha

   Plasmid

   #175939

   Purpose

   knockout mouse Ldha

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Ldha gRNA (Ldha Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAL-p300-1000-1600

   Plasmid

   #89101

   Purpose

   mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 1000-1600

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAL-p300-2-300

    Plasmid

    #89098

    Purpose

    mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 2-300

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-7 aSyn C141

    Plasmid

    #108866

    Purpose

    Expresses human WT alpha synuclein with a C-terminal cysteine

    Depositor

    Gabriele Kaminski Schierle

    Article

    Stephens et al bioRxiv 2022.10.04.510646

    Insert

    alpha synuclein (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    C141 mutation to allow C-terminal maleimide dye l…

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2plus-mKate2

    Plasmid

    #162624

    Purpose

    Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mKate2
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GAL-p300-1901-2414

    Plasmid

    #89103

    Purpose

    mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1901-2414

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX317-RBFOX1

    Plasmid

    #115442

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    RBFOX1 (RBFOX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Tags

    HA

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. EGFP-IpgD

    Plasmid

    #183666

    Purpose

    N-terminally tagged Shigella flexneri IpgD for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-MED22

    Plasmid

    #15423

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED22 (MED22 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only