We narrowed to 11,743 results for: pcdna

1. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-His-Max-A-PQBP1-Y65C

   Plasmid

   #25803

   Depositor

   Marius Sudol

   Article

   Tapia et al J Biol Chem. 2010 Apr 21. ():.

   Insert

   PQBP1 with Y65C mutation (PQBP1 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Tyrosine 65 within the WW domain of PQBP1 is chan…

   Promoter

   Available since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-mouse PKA-Calpha-mPAGFP

   Plasmid

   #45524

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA catalytic subunit alpha tagged by mPAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO-EGFP-G3BP1-S149A

   Plasmid

   #136004

   Purpose

   DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S149A

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

   Plasmid

   #136005

   Purpose

   DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-term of G3BP1 Deleted for Delta RBP

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mas-GRK2RH-Nluc L118A

   Plasmid

   #158606

   Purpose

   Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-N-mSOG-bFos-IRES-mCherry

   Plasmid

   #136599

   Purpose

   Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-bFos
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

   Plasmid

   #18016

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13V Venus-Y67G
   

   Use

   Tags

   ECFP and Venus-Y67G

   Expression

   Mammalian

   Mutation

   mglB-D154A; non-functional Venus

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p6042 pcDNA-Xpress-His-USP5

    Plasmid

    #24744

    Depositor

    Peter Howley

    Article

    Vos et al J Virol. 2009 Sep . 83(17):8885-92.

    Insert

    USP5 (USP5 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

    Plasmid

    #171429

    Purpose

    Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C261
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-HA-HA-human CMYC (170-439)

    Plasmid

    #203430

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA8-Flag-LARS(721-1176aa)

    Plasmid

    #139690

    Purpose

    Expresses N-teminal Flag tagged aa 721-1176 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid

    #123339

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1121 pcDNA3 GFP FKHR H215R

    Plasmid

    #9027

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid

    #54469

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

    Plasmid

    #171785

    Purpose

    Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO/myc-His-A-YAP2-S127A

    Plasmid

    #19062

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 127 changed to Alanine

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

    Plasmid

    #83461

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, R4113A

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-LifeAct-FRB-Venus-173-N

    Plasmid

    #87616

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-Venus173-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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