We narrowed to 11,284 results for: VARS

1. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT4-U6-sgRNA-CMV-EGFP

   Plasmid

   #239587

   Purpose

   Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   Sleeping beauty transposone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX303-PCDH7-a

   Plasmid

   #108538

   Purpose

   Expresses human PCDH7 transcript variant a in mammalian cells

   Depositor

   Kathryn O’Donnell

   Article

   Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

   Insert

   PCDH7 transcript variant a (PCDH7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_RHEB_WT

   Plasmid

   #81257

   Purpose

   Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 SARS-CoV-2 S D614G

   Plasmid

   #158075

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWXPGR-pTF-dnTCF4

   Plasmid

   #114277

   Purpose

   TET-inducible expression of a dominant negative form of the TCF4 transcription factor

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Lentiviral

   Mutation

   deletion of AA 1 to 31

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-YBX1

    Plasmid

    #192904

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    YBX1 (YBX1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106180

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV5

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-BLAST TFR1

    Plasmid

    #169890

    Purpose

    Express TFR1 open reading frame. Lacks IRE regulatory elements

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    TFRC (TFRC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    TFRC cDNA, lacking 3'UTR regulatory elements

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hSyn-ASAP4b-Kv-WPRE

    Plasmid

    #201030

    Purpose

    Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4b-Kv
    

    Use

    AAV

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

    Plasmid

    #165082

    Purpose

    gRNA 1 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS2280

    Plasmid

    #111901

    Purpose

    ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple345 (NEFL Human)
    

    Use

    AAV

    Promoter

    Ple345 (NEFL MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TYRO3-V5/HIS

    Plasmid

    #200118

    Purpose

    Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human TYRO3 receptor tyrosine kinase (TYRO3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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