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CD4d3+4-blac

Plasmid

#32403

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Type

Empty backbone

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Tags

HA

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pENTR-COIL

Plasmid

#16172

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Coilin (COIL Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
IDG_TMEM63B_OE_1

Plasmid

#161682

Purpose

Overexpresses TMEM63B

Depositor

Mike McManus

Insert

TMEM63B (TMEM63B Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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BC1177-pHR-SFFV-GFP(1-10)-NLS

Plasmid

#164039

Purpose

Expression of GFP1-10 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

GFP(1-10)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Nucbow

Plasmid

#158992

Purpose

Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Nucbow

Tags

All FPs are fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f1

Plasmid

#158755

Purpose

Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f1

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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co luxC pcDNA3.1(+)

Plasmid

#129087

Purpose

Expression of luxC in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxC

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co luxD pcDNA3.1(+)

Plasmid

#129088

Purpose

Expression of luxD in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxD

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co frp pcDNA3.1(+)

Plasmid

#129090

Purpose

Expression of frp in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co frp

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-mFoxJ1

Plasmid

#122965

Purpose

Expresses Flag-tagged mouse FoxJ1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

mouse FoxJ1 (Foxj1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-PAFAH1B1

Plasmid

#92430

Purpose

mammalian expression of PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_150/pcDNA3

Plasmid

#51984

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-HA-SrtAstrep (pKS77)

Plasmid

#50030

Purpose

Expression of cytosolic S. pyogenes SrtA in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Tags

HA

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO-shTRAF2#2

Plasmid

#44128

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
V5-LOV-Turbo1-G3BP1_pTRE-G418

Plasmid

#202015

Purpose

expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-G3BP1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-nucl

Plasmid

#113915

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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