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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,009 results for: ADA

Showing: 5921 - 5940 of 10009 results

  2. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  3. pDisplay-EGFP-CAAX

    Plasmid
    #208683
    Purpose
    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208683-plasmid-map-sequence-id-412176

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  4. p2R3a-Tq2CFP-OcsT

    Plasmid
    #71268
    Purpose
    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Tq2CFP
    Use
    Gateway
    Tags
    4xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71268-plasmid-map-sequence-id-129804

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  5. p2R3a-GUS-OcsT

    Plasmid
    #71267
    Purpose
    Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Beta-glucuronidase
    Use
    Gateway
    Tags
    2xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71267-plasmid-map-sequence-id-129805

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  6. pDisplay-GRAB_CRFmut

    Plasmid
    #208656
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208656-plasmid-map-sequence-id-410341

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  10. qTAG-N-Solo-mStayGold-CETN2

    Plasmid
    #227292
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227292-plasmid-map-sequence-id-452065

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  16. qTAG-C-Solo-mStayGold-CANX

    Plasmid
    #227280
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227280-plasmid-map-sequence-id-452047

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  19. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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Showing: 5921 - 5940 of 10009 results