Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,996 results for: SHI

Showing: 5921 - 5940 of 13996 results

Results per page:

Sort results by:

pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(POU5F1_g1)-PGKpuroBFP-W

Plasmid

#211980

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(POU5F1_g15-14)-PGKpuroBFP-W

Plasmid

#211981

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(ETV4_5-1)-PGKpuroBFP-W

Plasmid

#211960

Purpose

Express gRNA against ETV4 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETV4 (ETV4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(ETV4_5-2)-PGKpuroBFP-W

Plasmid

#211961

Purpose

Express gRNA against ETV4 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETV4 (ETV4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(JARID2_5-5)-PGKpuroBFP-W

Plasmid

#211967

Purpose

Express gRNA against JARID2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting JARID2 (JARID2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO shLuc/FLAG Metap2

Plasmid

#209316

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 human, Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO shLYCHOS/LYCHOS WT-FLAG

Plasmid

#209315

Purpose

Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO shLYCHOS/FLAG Metap2

Plasmid

#209314

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-CG-LYCHOS FP>IA-HA-GFP

Plasmid

#199688

Purpose

Expression of LYCHOS FP>IA mutant in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

10xHis, 6xHis, EGFP, HA, and HRV 3C

Expression

Insect

Mutation

F43 and P44 mutated to I and A respectively

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a LZ LED 4CA-FLAG

Plasmid

#199681

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED 4CA (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

C595.604.629.638 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a LZ LED Y551A-FLAG

Plasmid

#199680

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED Y551A (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Y551 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD 3R-3E

Plasmid

#188148

Purpose

Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A

Plasmid

#188134

Purpose

Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD R1403A R1404A

Plasmid

#188135

Purpose

Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD L1405A

Plasmid

#188136

Purpose

Expresses C-terminal flag-tagged human CAD L1405A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD A1400E

Plasmid

#188137

Purpose

Expresses C-terminal flag-tagged human CAD A1400E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD C1374S

Plasmid

#188139

Purpose

Expresses C-terminal flag-tagged human CAD C1374S in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1367A

Plasmid

#188140

Purpose

Expresses C-terminal flag-tagged human CAD E1367A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1373A

Plasmid

#188142

Purpose

Expresses C-terminal flag-tagged human CAD E1373A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5921 - 5940 of 13996 results

Results per page:

Sort results by: