We narrowed to 9,384 results for: Coli
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Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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pMD19T-slr0230-PL31-sgRNANT1-KmR
Plasmid#73221PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL31-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL31Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH09
Plasmid#70064Purposeartificial transcription template "terminator insertion"DepositorInsertlambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p70rg 5
Plasmid#17829DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMK83
Plasmid#226793PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10BDepositorInsertslr0214 (slr0214 Synechocystis sp. PCC 6803)
UseTagsExpressionBacterialMutationPromoterTetracycline resistance cassette promoter (consti…Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_K262A
Plasmid#225728PurposeTo express Mce3R mutant K262A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationK262APromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelG
Plasmid#225729PurposeTo express MelG (Rv1937) in E. coliDepositorInsertmelG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-VapBC35
Plasmid#225733PurposeTo express VapBC35(Rv1962) Toxin antitoxin in E. coliDepositorInsertvapBC35
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T_Mce3R
Plasmid#225726PurposeTo express Mce3R in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PHOSPHO2-TEV-Twin-Strep
Plasmid#222017PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coliDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionBacterialMutationdeleted stop codon (TAA)Promoterlac promoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-yfp
Plasmid#227641PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_R53A
Plasmid#225727PurposeTo express Mce3R mutant R53A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationR53APromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Ealbertii
Plasmid#223360PurposeEncoding ClbS from Escherichia albertii CB9786DepositorInsertEncoding ClbS from Escherichia albertii CB9786 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Frischella
Plasmid#223358PurposeEncoding ClbS from Frischella perraraDepositorInsertEncoding ClbS from Frischella perrara (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Snod
Plasmid#223355PurposeEncoding ClbS from Snodgrassella alvi wkB2DepositorInsertEncoding ClbS from Snodgrassella alvi wkB2 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Mixta
Plasmid#223352PurposeEncoding ClbS from Mixta theicolaDepositorInsertEncoding ClbS from Mixta theicola (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Dickeya
Plasmid#223351PurposeEncoding ClbS from Dickeya dadantiiDepositorInsertEncoding ClbS from Dickeya dadantii (Codon optimized for E coli) (CO076_RS11420 Dickeya dadantii)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-TrxC
Plasmid#225742PurposeTo express TrxC(Rv3914) in E. coliDepositorInsertTrxC
UseTags6xHisExpressionBacterialMutationTrxCPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only