We narrowed to 40,132 results for: ANT

1. pKan cpRBP-FL P53

   Plasmid

   #174504

   Purpose

   Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

   Depositor

   Stewart Loh

   Article

   Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

   Insert

   P53 gene (TP53 Human)
   

   Tags

   20 amino acid linker containing HRV3C protease si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128587

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pαH-S-GSAS-B.1.1.7

   Plasmid

   #171747

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3Ai E72A-Cas9n-UGI-NLS

   Plasmid

   #109430

   Purpose

   Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus2-DeltaCT-STAT3

   Plasmid

   #123179

   Purpose

   Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Deletion of the SH2 and TAD domains (C-terminus, …

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #108273

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α promoter (1.1kb short version)

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus1-DeltaCT-STAT3

   Plasmid

   #123178

   Purpose

   Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   Deletion of the SH2 and TAD domains (C-terminus, …

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #118295

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #128588

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)

    Plasmid

    #160646

    Purpose

    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S, 35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-GSAS-B.1.351

    Plasmid

    #171749

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-TR-hCAII

    Plasmid

    #232480

    Purpose

    Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Human carbonic anhydrase II (CA2 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHLsec hSOD1

    Plasmid

    #232481

    Purpose

    vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304-BCL-XL-499/500R

    Plasmid

    #203575

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    silent point mutations to make resistant shRNA 49…

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-chimeric-IgM-mouse/human_M18

    Plasmid

    #91738

    Purpose

    Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Residues 203-239 (EU numbering) were exchanged to…

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-CuO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-TR-hSOD1

    Plasmid

    #232478

    Purpose

    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCRW1

    Plasmid

    #122462

    Purpose

    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

    Depositor

    Richard C. Willson

    Article

    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

    Insert

    NPM-ALK fusion protein (ALK, NPM1 Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7lac promoter

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid

    #109429

    Purpose

    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGL4.11-COL1A1mTEAD

    Plasmid

    #230916

    Purpose

    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase- luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    mutant potential TEAD bind elements AGGAAT to CTG…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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