We narrowed to 117,675 results for: MPI;

1. U6+27-16

   Plasmid

   #21975

   Depositor

   Phil Sharp

   Article

   Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

   Insert

   U6 miR-16 sponge
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pHH0103 SMURF2 WW domain #3

   Plasmid

   #104226

   Purpose

   Bacterial expression of WW domain #3 from SMURF2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   SMURF2 WW domain #3 (SMURF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103 SMURF2 WW domain #2

   Plasmid

   #104225

   Purpose

   Bacterial expression of WW domain #2 from SMURF2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   SMURF2 WW domain #2 (SMURF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103 SMURF2 WW domain #1

   Plasmid

   #104224

   Purpose

   Bacterial expression of WW domain #1 from SMURF2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   SMURF2 WW domain #1 (SMURF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBS.EF

   Plasmid

   #129335

   Purpose

   Lentivirus vector

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   unspecified
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW8-aid-V5-eDSR-Turg1:kanMX6

   Plasmid

   #169365

   Purpose

   C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   1XV5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW8-aid-5Myc-Turg1:kanMX6

   Plasmid

   #169356

   Purpose

   C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   5XMyc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP-RAB11A

   Plasmid

   #25120

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PF13_0119:C34946
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Nup133_mEGFP(-9a)

   Plasmid

   #163419

   Purpose

   Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)

   Depositor

   Sanford Simon

   Insert

   Nup133 (NUP133 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-GFP-miRE_shRen-Blast

    Plasmid

    #135684

    Purpose

    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CMV-tdTomato-miRE_shRen-Blast

    Plasmid

    #135686

    Purpose

    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

    Plasmid

    #55212

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 69, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UC20m

    Plasmid

    #121040

    Purpose

    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).

    Depositor

    Richard Murray

    Insert

    gN2 Cas9 gRNA UC part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid

    #137859

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1-tax-4P::his-24::mOrange

    Plasmid

    #124340

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    tax-4, mOrange (tax-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pE-SUMO CRYGB

    Plasmid

    #80751

    Purpose

    Expression of human gamma B-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGB (CRYGB Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS chick LH-2A [TJ#93]

    Plasmid

    #16280

    Depositor

    Thomas Jessell

    Insert

    LH-2A (LHX2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

18. L4752

    Plasmid

    #1675

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mouse NCKX2-AS-long (pcDNA3.1+)

    Plasmid

    #75199

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Insert

    SLC24A2 (Slc24a2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T12m

    Plasmid

    #121031

    Purpose

    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ECK120029600 (strongest natural Voigt terminator)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge