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pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

Plasmid

#115518

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

AAV; Donor plasmid for recombinase-mediated casse…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-kanMX6

Plasmid

#18820

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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tandem PA-GFP-KanR

Plasmid

#37548

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

tandem PA-GFP

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-His3MX6

Plasmid

#18822

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-TRP1

Plasmid

#18821

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Stop-EGFP (MSE)

Plasmid

#154469

Purpose

MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)

Depositor

Cornelius Miething

Article

Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pABCb10aa1-35-GFP

Plasmid

#19035

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1-35 of the mitochondrial targeting sequence of ABCb10

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pL3-TRE-LucGFP-2L

Plasmid

#11685

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Insert

Luciferase-EGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pdelta105-ABCb10-GFP

Plasmid

#19034

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

ABCb10 lacking the mitochondrial targetting sequence

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
GFP H2B SL2

Plasmid

#227718

Purpose

Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

GFP H2B SL2 (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SL2 GFP H2B

Plasmid

#227717

Purpose

Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

SL2 GFP H2B (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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T2A 3xNLS GFP

Plasmid

#227721

Purpose

Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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cEF1a-GFP

Plasmid

#135671

Purpose

Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

Depositor

Florian Karreth

Insert

GFP

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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cSFFV-GFP

Plasmid

#135670

Purpose

Introduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

Depositor

Florian Karreth

Insert

GFP

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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AICSDP-63: ACTN2-mEGFP

Plasmid

#124607

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Insert

ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-BsaI_pJMP2748

Plasmid

#208888

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Pir dependent origin of replication

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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