We narrowed to 344 results for: 1266

1. EF1a-TALEN_NN (SPC left)

   Plasmid

   #126694

   Purpose

   left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   human SPC left
   

   Use

   TALEN

   Tags

   0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-DR-cr in PBCSK-

   Plasmid

   #126641

   Purpose

   Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FnoCas12a-2C-NLS in pCSDest

   Plasmid

   #126639

   Purpose

   Expresses FnoCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-bsd-Flag-Trl1

   Plasmid

   #126609

   Purpose

   Expresses N-terminal Flag-tagged yeast Trl1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA ligase 1 (TRL1 Budding Yeast)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT Lifeact-TagBFP

   Plasmid

   #126664

   Purpose

   Inducible expression of Lifeact-TagBFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Lifeact-TagBFP fusion
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOSV812

   Plasmid

   #126605

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOSV808

   Plasmid

   #126601

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Src42a

   Plasmid

   #126673

   Purpose

   Expression of GST-Src42a for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Src42a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE2-RSV-jGCaMP7s-W

   Plasmid

   #126690

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7s
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Expression

   Mutation

   A52V, A317L

   Promoter

   RSV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-puro-V5-Trpt1

    Plasmid

    #126607

    Purpose

    Expresses N-terminal V5-tagged mouse Trpt1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOSV810

    Plasmid

    #126603

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT Draper-mCherry

    Plasmid

    #126667

    Purpose

    Inducible expression of Draper-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    C813Y- see depositor comments below

    Promoter

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOSV806

    Plasmid

    #126606

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2-RSV-jGCaMP7f-W

    Plasmid

    #126691

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7f
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    H78K, A317L

    Promoter

    RSV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT p85-GFP

    Plasmid

    #126660

    Purpose

    Inducible expression of p85-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    PI3K SH2 containing subunit
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TrmD_HaeIn/pET28b(+) N,C-tag

    Plasmid

    #12665

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-hSPC-TFEBeGFP-W

    Plasmid

    #126692

    Purpose

    Lineage specific over expression of TFEB in lung alveolar epithelial cells

    Depositor

    Darrell Kotton

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted Stop Codon on TFEB

    Promoter

    hSPC

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FnoCas12a-DR-cr in PBCSK-

    Plasmid

    #126642

    Purpose

    Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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