We narrowed to 107 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Publication

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1761 Articles

   

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These results may also be relevant.

1. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Publication

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN243-EN1953

   Plasmid

   #224546

   Purpose

   Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

   Depositor

   Elphege Nora

   Publication

   Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)
   

   Expression

   Mammalian

   Available since

   Dec. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Expression

   Mammalian

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPH2A.X_S139D-GS3

   Plasmid

   #204748

   Purpose

   Expression of Cas9 and human H2AX with the S139D mutation

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   H2AX S139D (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the S139D mutation

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sgRNA2-CTCF-prom

   Plasmid

   #195104

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Publication

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sgRNA3-RPB1-N-term

   Plasmid

   #195112

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Publication

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-sgRNA1-RPB1-N-term

   Plasmid

   #195110

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Publication

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX459-sgRNA2-2-RPB1-C-term

   Plasmid

   #195109

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Publication

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   sgRNA against last exon of RPB1 (C-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sgRNA4-CTCF-3p-UTR

   Plasmid

   #195106

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Publication

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   sgRNA against CTCF 3' UTR region
   

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459-sgRNA3-CTCF-3p-UTR

    Plasmid

    #195105

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Publication

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    sgRNA against CTCF 3' UTR region
    

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124286

    Purpose

    sgRNA against human Col7A1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGB

    Plasmid

    #204744

    Purpose

    gRNA-Bsd expression vector

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rab11 single guide RNA

    Plasmid

    #229683

    Purpose

    guide RNA for Rab11 tagging at the N-terminus in pX459

    Depositor

    Francesca Bottanelli

    Publication

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    Rab11 guide (RAB11A Human)
    

    Use

    CRISPR

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

    

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14. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Publication

    Lucero et al bioRxiv, March 19, 2025

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Expression

    Mammalian

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Publication

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Publication

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-sgRNA1-CTCF-prom

    Plasmid

    #195103

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Publication

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    sgRNA against CTCF promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX462-ABE8e-nSaCas9

    Plasmid

    #237462

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Publication

    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

    Insert

    3xFlag_ABE8e_SaCas9 -6xNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX461-ABE8e-nSpCas9

    Plasmid

    #237460

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Publication

    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

    Insert

    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX461-evoCDA1-nSpCas9

    Plasmid

    #237461

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Publication

    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

    Insert

    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge