We narrowed to 58 results for: fucci

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pMIR218-2_nt5G>U

Plasmid

#174009

Purpose

miR218 nt5 point mutation in SLIT3

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

SLIT3 (SLIT3, MIR218-2 Human)

Expression

Mammalian

Mutation

NT5 G>U point mutation in SLIT3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

Plasmid

#176932

Purpose

Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

Plasmid

#191527

Purpose

Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

CRISPR and Synthetic Biology

Tags

mNeonGreen (mNG) + 3xFLAG_TwinStrep

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Hygro-hTNNT2-FUUCIplex

Plasmid

#240220

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The hTNNT2 promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral

Expression

Mammalian

Promoter

hTNNT2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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MultiMate-Cell-cycle

Plasmid

#206280

Purpose

Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B iRFP713, mAG-hGeminin, mKO2-hCdt1

Use

Recombinant baculovirus production

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-CAR

Plasmid

#157743

Purpose

knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

Plasmid

#157744

Purpose

knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRetro-CAG-DsRedExpress-TVA

Plasmid

#235699

Purpose

Control plasmid expressing TVA and DsRed without G protein

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

Plasmid

#92139

Purpose

Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRetro-CAG-DsRedExpress-G-TVA

Plasmid

#235698

Purpose

Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800, and Glyco

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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