We narrowed to 1,134 results for: fret

1. pCSIIbsr-Raichu-Rab5/PM

   Plasmid

   #214819

   Purpose

   Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.

   Depositor

   Michiyuki Matsuda

   Article

   Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

   Insert

   A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
   

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-rsAKARev(T/A)

   Plasmid

   #166232

   Purpose

   Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

   Depositor

   Peter Dedecker

   Article

   Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

   Insert

   Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
   

   Expression

   Mammalian

   Mutation

   changed Threonine 506 in rsAKARev to Alanine

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-CytERM-N-17

   Plasmid

   #55895

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   CytERM
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pmVenus(L68V)-mTurquoise2

   Plasmid

   #60493

   Purpose

   Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   SYFP2-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RalGDS-YPet

   Plasmid

   #134929

   Purpose

   Fluorescently tagged affinity reagent

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   RaIGDS RBD, YPet
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRuby2-ZO1-C-14

   Plasmid

   #55916

   Purpose

   Localization: Tight Junctions, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAuroraA-GFP-AURKA_K162M-mCherry

    Plasmid

    #99880

    Purpose

    Expression of GFP-AURKA K162M-mCherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    K162M (kinase-dead)

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. peCALWY-4

    Plasmid

    #22236

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)
    

    Expression

    Mammalian

    Mutation

    C418S mutation linker consists of 9 GGSGGS repea…

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCerulean3-Rap1

    Plasmid

    #134928

    Purpose

    Fluorescently tagged GTPase.

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1B, Cerulean
    

    Expression

    Mammalian

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Shp2 biosensor

    Plasmid

    #134346

    Purpose

    FRET biosensor. To visualize Shp2 activity by FRET

    Depositor

    Yingxiao Wang

    Article

    Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

    Insert

    Shp2 Biosensor
    

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rap1A FLARE

    Plasmid

    #134926

    Purpose

    Rap1A biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1A, mCerulean3, RaIGDS RBD, YPet
    

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-GFP-AURKA_K162M-mCherry

    Plasmid

    #99881

    Purpose

    Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    K162M (kinase-dead)

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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