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Showing: 1451 - 1500 of 2288 results

  1. pGL3-pSMANTIS_1kb_1 KLF2mut 449,732

    Plasmid
    #194200
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194200-plasmid-map-sequence-id-381961

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  2. pGL3-pSMANTIS_1kb_1 KLF2mut 449,884

    Plasmid
    #194201
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194201-plasmid-map-sequence-id-381985

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  5. pBS513 EF1alpha-cre

    Plasmid
    #11918
    Purpose
    Expresses Cre under the control of the elongation factor-1 alpha (EF-1 alpha) promoter; includes upstream Kozak modification to increase expression.
    Depositor
    Brian Sauer
    Article
    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.
    Insert
    cre (cre bacteriophage P1)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1 alpha
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    11918-plasmid-map-sequence-id-163454

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  6. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

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  7. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  8. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    PAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

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  15. pcDNA3.1-Beta2-PAmcherry1

    Plasmid
    #114190
    Purpose
    studying clustering of Beta2 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Adrenergic receptor beta 2 (ADRB2 Human)
    Use
    Tags
    3HA, FLAG, and PAmcherry1
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114190-plasmid-map-sequence-id-224309

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  16. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

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  17. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

    Plasmid
    #107845
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in four clusters in spl…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107845-plasmid-map-sequence-id-203697

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  18. pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

    Plasmid
    #107844
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in two clusters in spli…
    Promoter
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107844-plasmid-map-sequence-id-203696

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  20. pIA036

    Plasmid
    #200425
    Purpose
    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    200425-plasmid-map-sequence-id-443100

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  22. pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]

    Plasmid
    #107846
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in six clusters in spli…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107846-plasmid-map-sequence-id-203698

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  29. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  43. HOXA1 (murine) HIS-tag pET

    Plasmid
    #8556
    Depositor
    Corey Largman
    Insert
    HOXA1 (Hoxa1 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    clone contains 130 AA including the homeodomain, …
    Promoter
    Available since
    July 5, 2006
    Availability
    Academic Institutions and Nonprofits only

  46. pGL3-basic-hFX-promotor(2kb, distal)

    Plasmid
    #14981
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    includes distal fragment of human frataxin promot…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  47. pLSLGUS

    Plasmid
    #51517
    Purpose
    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GUS
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51517-plasmid-map-sequence-id-78696

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  50. pLSLT

    Plasmid
    #51498
    Purpose
    pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    T30 TALEN pair
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51498-plasmid-map-sequence-id-78599

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Showing: 1451 - 1500 of 2288 results