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We narrowed to 4,045 results for: golden gate

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Showing: 1451 - 1500 of 4045 results

  1. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  2. C103m

    Plasmid
    #121007
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121007-plasmid-map-sequence-id-236162

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  4. pATUBR

    Plasmid
    #245488
    Purpose
    Binary Plasmid for plant transformation expressing RUBY from the Arabidopsis thaliana polyubiquitin 10 gene promoter and the terminator from the pea RBCS gene. Kanamycin plant selection
    Depositor
    Beth Rowan
    Insert
    pAtUBI-RUBY-tRBCS
    Expression
    Plant
    Promoter
    Polyubiquitin 10 gene from Arabidopsis thaliana
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245488-plasmid-map-sequence-id-487914

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  5. pAttBFHyg2

    Plasmid
    #244927
    Purpose
    attB Hyg Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion vector
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244927-plasmid-map-sequence-id-486008

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  6. pAttBFHyg

    Plasmid
    #244926
    Purpose
    attB Hyg Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244926-plasmid-map-sequence-id-486007

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  7. pAIP4_2ESK

    Plasmid
    #234232
    Purpose
    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2ESK (UBE2D2 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234232-plasmid-map-sequence-id-464196

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  8. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  9. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Tags
    HisTag
    Promoter
    T7
    Available Since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

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  12. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

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  13. pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3

    Plasmid
    #242154
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P1.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242154-plasmid-map-sequence-id-492357

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  14. pDest_PANCEd_AraC_R2_S170_LOV_P2.1_C3

    Plasmid
    #242155
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P2.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P2.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, E144K, R146L, A152T, S225R, R251L; AsL…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242155-plasmid-map-sequence-id-492368

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  15. pRDB_031

    Plasmid
    #245331
    Purpose
    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
    Depositor
    John Doench, David Root
    Insert
    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245331-plasmid-map-sequence-id-488051

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  16. pRDB_245

    Plasmid
    #245332
    Purpose
    Cas12a CRISPRko positive control guide; targets CD46
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245332-plasmid-map-sequence-id-488043

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  17. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

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  18. pRDB_247

    Plasmid
    #245334
    Purpose
    Cas12a CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245334-plasmid-map-sequence-id-488032

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  19. pMV_AA097

    Plasmid
    #245319
    Purpose
    Cas12a CRISPRko positive control; targets CD46, CD47, CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46, CD47, CD55 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245319-plasmid-map-sequence-id-488024

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  20. pDest_PANCEd_AraC_R2_LDelK

    Plasmid
    #242156
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE/RAMPhaGE Linker Library variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV LDelK
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L; Left Linker: GS -…
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    242156-plasmid-map-sequence-id-486033

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  21. pAIP4_6WEY

    Plasmid
    #234234
    Purpose
    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234234-plasmid-map-sequence-id-464198

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  22. pAIP4_3D1B

    Plasmid
    #234230
    Purpose
    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3D1B (tas3 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234230-plasmid-map-sequence-id-464195

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  23. pAIP4_5F66

    Plasmid
    #234226
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5F66 (PPIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234226-plasmid-map-sequence-id-464191

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  24. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  25. pAIP4_5EIP

    Plasmid
    #234188
    Purpose
    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EIP (mmi1 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234188-plasmid-map-sequence-id-464153

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  26. pAIP4_5KUW

    Plasmid
    #234183
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5KUW (PPIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234183-plasmid-map-sequence-id-464148

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  27. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  28. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  29. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  30. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  31. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

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  32. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

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  33. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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  34. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid
    #219711
    Purpose
    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219711-plasmid-map-sequence-id-435738

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  35. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

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  36. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

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  37. pK7-g7g8-K8

    Plasmid
    #218220
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218220-plasmid-map-sequence-id-432226

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  38. pK3-g3g4-K4

    Plasmid
    #218218
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218218-plasmid-map-sequence-id-432228

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  39. pK5-g5g6-K6

    Plasmid
    #218219
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218219-plasmid-map-sequence-id-432227

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  40. pAttPF3Hyg

    Plasmid
    #244929
    Purpose
    attP Hyg Resistant plasmid for RMI targeting an attB site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244929-plasmid-map-sequence-id-486014

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  41. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  42. pAIP4_6IYE

    Plasmid
    #234237
    Purpose
    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234237-plasmid-map-sequence-id-464201

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  43. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

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  44. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  45. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219687
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219687-plasmid-map-sequence-id-435722

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  47. pMB.MBP.JARID2

    Plasmid
    #247018
    Purpose
    Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease)
    Depositor
    Chen Davidovich
    Insert
    JARID2 (JARID2 Human)
    Tags
    MBP
    Expression
    Insect
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247018-plasmid-map-sequence-id-493374

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  48. pLSUBR

    Plasmid
    #245489
    Purpose
    Binary Plasmid for plant transformation expressing RUBY from the Lactuca sativa polyubiquitin 4 gene promoter and the terminator from the same gene. Kanamycin plant selection
    Depositor
    Beth Rowan
    Insert
    pLsUBI-RUBY-tLSUBI
    Expression
    Plant
    Promoter
    Polyubiquitin 4 gene (LOC111919935) from lettuce
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245489-plasmid-map-sequence-id-488936

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  49. pMV_AA100

    Plasmid
    #245322
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD55
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245322-plasmid-map-sequence-id-488050

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  50. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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