We narrowed to 585 results for: 1305

1. pJC45BS104 Y507W

   Plasmid

   #1305

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 Y507W (HSP104 Budding Yeast)
   

   Tags

   10X His tag

   Expression

   Bacterial

   Mutation

   Changed Tyr 507 to Trp

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pHEE2E-TRI

   Plasmid

   #71288

   Purpose

   Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA targeting TRY and CPC genes
   sgRNA targeting ETC2 gene
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
   TRAC-3' homology arm (TRAC Human)
   

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiT3.1-N_WDR62:1-1518

   Plasmid

   #211257

   Purpose

   Mammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR62:M1-H1518 (WDR62 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   isoform 4. L850S natural VAR_031299. Q1305L n…

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge