Addgene

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pSmart-EF1alpha-NlaCas7-p65

Plasmid

#207394

Purpose

Expresses NlaCas7-p65 activator fusion in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-NlaCas7-p65-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 heavy chain

Plasmid

#198253

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-T7-CBD-hGLY

Plasmid

#134940

Purpose

T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

t7

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-CBD-hGLY

Plasmid

#134596

Purpose

Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCLTC v2 puro

Plasmid

#192348

Purpose

Lentiviral expression vector for an inducible shCLTC v2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCLTC v2 (CLTC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCLTC v1 puro

Plasmid

#192347

Purpose

Lentiviral expression vector for an inducible shCLTC v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCLTC v1 (CLTC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-CHC17KDP

Plasmid

#59799

Purpose

Expresses knockdown-proof human CHC17 tagged with GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

CHC17 (CLTC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 61-65 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG1_M18

Plasmid

#82357

Purpose

Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro-CLTC

Plasmid

#227314

Purpose

Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-CLTC

Plasmid

#227313

Purpose

Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_M18

Plasmid

#82356

Purpose

Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_CLTC_Clathrin propeller

Plasmid

#178471

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLTC_Clathrin propeller (CLTC Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_CLTC_Clathrin

Plasmid

#109997

Purpose

Protein expression and purification of CLTC_Clathrin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CLTC_Clathrin (CLTC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

Plasmid

#214950

Purpose

Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion

Use

Tags

6xHis, Amino half of venus BIFC fragment, Carboxy…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-SRRD-mRuby3

Plasmid

#214920

Purpose

inducible expression of SRRD tagged on C-terminus with mRuby3

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-noTag (in pLEX_307)

Plasmid

#214919

Purpose

constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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